Assessment of three-dimensional biofilm structure using an optical microscope.

نویسندگان

  • Carla C C R de Carvalho
  • M Manuela R da Fonseca
چکیده

A method allowing the evaluation of the structure and the calculation of the volume of a biofilm, using an optical microscope, is proposed based on the linear relation between the intensity of a pixel in biofilm images grabbed on the x-y plane and the corresponding number of cells in the z direction, which allows the calculation of the biofilm thickness. The method is intended to overcome the need for expensive microscopes to study biofilms.

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عنوان ژورنال:
  • BioTechniques

دوره 42 5  شماره 

صفحات  -

تاریخ انتشار 2007